General Information

  • ID:  hor003120
  • Uniprot ID:  A0SIF1
  • Protein name:  RLRW peptide 2
  • Gene name:  sNPF
  • Organism:  Anopheles gambiae (African malaria mosquito)
  • Family:  NPY family
  • Source:  Animal
  • Expression:  Expressed in all body parts of larva, pupae and adults.
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  gambiae species complex, Pyretophorus, Cellia (subgenus), Anopheles (genus), Anophelinae (subfamily), Culicidae (family), Culicoidea (superfamily), Culicomorpha (infraorder), Nematocera (suborder), Diptera (order), Endopterygota (cohort), Neoptera (infraclass), Pterygota (subclass), Dicondylia, Insecta (class), Hexapoda (subphylum), Pancrustacea, Mandibulata, Arthropoda (phylum), Panarthropoda, Ecdysozoa, Protostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  GO:0005184 neuropeptide hormone activity
  • GO BP:  GO:0007218 neuropeptide signaling pathway; GO:0032095 regulation of response to food; GO:0040014 regulation of multicellular organism growth
  • GO CC:  GO:0005576 extracellular region

Sequence Information

  • Sequence:  APTQRLRW
  • Length:  8(197-204)
  • Propeptide:  MYRINLTTFTLLLVLAVGSLMSESLHPSDGAINDLYEYLLQREYAAPVSYADHQIKRKAVRSPSLRLRFGRRSDPSVPLRPEEDELIDQKAIRAPQLRLRFGRNDPLWTSFNENALLEENFEKRAPSQRLRWGRSNLFGNLVNQFQQDDVMQQKTIRAPQLRLRFGRTDPSWAMYNEHQLTTGQQAQPANEASEKRAPTQRLRWGRSDPALAKDSSEDKALDVEESENTNADDK
  • Signal peptide:  MYRINLTTFTLLLVLAVGSLMS
  • Modification:  T8 Tryptophan amide
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  Has a role in inhibiting host-seeking behavior during a reproductive cycle
  • Mechanism:  NA
  • Cross BBB:  NA
  • Target:  NA
  • Target Unid:   NA
  • IC50:  sNPF receptor:380nM
  • EC50:  NA
  • ED50:  NA
  • Kd:  NA
  • Half life:  NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  AF-Q9LUH9-F1(AlphaFold_DB_ID)

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    AF-Q9LUH9-F1.pdbhor003120_AF2.pdbhor003120_ESM.pdb

Physical Information

Mass: 115223 Formula: C46H74N16O11
Absent amino acids: CDEFGHIKMNSVY Common amino acids: R
pI: 12.5 Basic residues: 2
Polar residues: 1 Hydrophobic residues: 3
Hydrophobicity: -126.25 Boman Index: -2889
Half-Life / Aliphatic Index: 4.4 hour Aliphatic Index: 61.25
Instability Index: 11907.5 Extinction Coefficient cystines: 5500
Absorbance 280nm: 785.71

Literature

  • PubMed ID:  17140700
  • Title:  Characterization and Expression of the Short Neuropeptide F Receptor in the African Malaria Mosquito, Anopheles Gambiae